Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Summary of the antibodies used for immunohistochemistry of GCTs and schwannomas.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Immunohistochemistry, Cell Surface Receptor Assay, Membrane, Plasmid Preparation

Journal: Scientific Reports

Article Title: Expression and regulation of FRMD6 in mouse DRG neurons and spinal cord after nerve injury

doi: 10.1038/s41598-020-58261-7

Figure Lengend Snippet: Primary antibodies.

Article Snippet: GAP43 , Rabbit , IHC (Coons) , 1 μg/ml , Atlas Antibodies AB/HPA015600.

Techniques:

Journal: The Journal of Neuroscience

Article Title: Targeted Deletion of a Cyclic Nucleotide-Gated Channel Subunit (OCNC1): Biochemical and Morphological Consequences in Adult Mice

doi: 10.1523/JNEUROSCI.19-21-09313.1999

Figure Lengend Snippet: GAP43 immunostaining in the olfactory epithelium of wild-type (A, wt) and OCNC1-null (B, ko) mice. Fewer neurons (small arrows) are stained with GAP43, indicating that the number of both mature and immature neurons is lower in the null mice. The double arrows indicate the difference in the thickness of the olfactory epithelium (oe). lp, Lamina propria; nc, nasal cavity; s, septum. Scale bar, 30 μm.

Article Snippet: Afterwards, sections were incubated in either goat anti-OMP (1: 5000) or mouse anti-GAP43 (1:100; Boehringer Mannheim, Indianapolis, IN) overnight at room temperature.

Techniques: Immunostaining, Staining

Journal: Scientific Reports

Article Title: The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein G olf to Olfactory Cilia

doi: 10.1038/s41598-017-08543-4

Figure Lengend Snippet: Histological and subcellular distribution of LRBA in olfactory and vomeronasal tissues. ( A ) LacZ enzyme histochemistry of Lrba/gt gene-trap mice (age, 3 weeks) demonstrates expression of the LRBA gene throughout the OE (top). Larger magnification (middle) shows that the staining is predominantly localized to the OSNs. Respiratory epithelium (RE, bottom) is also stained, though weaker than OE. ( B ) β-galactosidase staining was also seen in VNO neuron somata and the microvillar layer of Lrba/gt mice (top, middle), and confirmed by LRBA-IF of adult WT tissue (bottom; SE, sensory epithelium; L, lumen). Scale bars in A , B : overviews 100 µm, close-ups 25 µm. ( C ) LacZ staining of the olfactory bulb required longer incubation (16 h) than of the OE and VNO (2 h). Scale bar in C : 100 µm. ( D – K ) Triple immunostaining for LRBA ( D , H ), OMP ( E , I ), GAP43 ( F , J ) and merged images ( G , K ) in different regions of the adult mouse OE of low ( D – G ) or high ( H–K ) thickness. LRBA-immunoreactivity (ir) is found in the apical somatic cytoplasm and in dendrites as well as dendritic knobs of virtually all OMP-ir mature OSNs. Large and small arrows point to select LRBA-/OMP-ir cell bodies/dendrites and dendritic knobs, respectively, but LRBA is lacking in the OMP-ir ciliary layer (small arrowheads in D – G ). GAP43-ir immature neurons contain no (large arrowheads in D – G ) or only small amounts (double arrows in D – K ) of LRBA-immunoreactivity in the apical cytoplasm of their cell bodies and in dendritic knobs. Scale bar in K for D – K : 10 µm. OE strata are designated in panels G and K: cl, cilial layer; scl, sustentacular cell layer; osnl, OSN layer.

Article Snippet: Antibodies used were rabbit anti-mLRBA-B (1:100), goat anti-OMP (1:300; Wako) as a marker for mature OSNs , and mouse anti-GAP43 (1:300; MerckMillipore) as a marker for immature OSNs , .

Techniques: Expressing, Staining, Incubation, Triple Immunostaining